THREE SIMPLE SIX · THE SCIENCE
A closer look
at the evidence.
Meet the reference entries, laboratory reports and manufacturing documents behind the conversation. Read what was studied, what was reported, and what remains to be established.
New to the Simple Six? Read A Great Place to Start.
Sources checked 25 October 2026 · Simple Six only
01 · REFERENCE LISTINGS
Every formula.
Its PDR label.
Manufacturer product-label entries and official THREE fact sheets, kept side by side.
Open Highlights of Prescribing Information on the PDR page for full product details. PDR links request a separate window; your browser may choose a tab. Product facts open here with original-source and download actions.
Éternel
Purifí
Imúne
Revíve
Vitalité
PDR pages reproduce manufacturer product-label information; they are not independent clinical proof, FDA approval or endorsement. Version note: Éternel’s PDR entry (2023 · 2301.V1.US-EN) describes an older fish-collagen-containing formula and includes apparent text errors. Compare its separate 2026 manufacturer fact sheet and current package label; do not treat that older entry as current ingredients or serving advice. Other historical label versions may also differ from current products.
02 · RESEARCH REPORTS
Start with the methods.
Keep the context.
These are laboratory findings in cell models. They are not trials of people taking today’s products, and historical formula versions have not been matched to current labels.
In-vitro Caco-2 permeability assay2023-06 (document code 2306.V1.US-EN; URL uploaded July 2023)
All six tested in an intestinal-cell model
The laboratory report found higher predicted absorption for each tested formula than for its curcumin control.
Study scope and limitations
- What was studied
- Tested Collagène, Éternel, Revíve, Purifí, Vitalité and Imúne; historical formula versions and lots unspecified.
- What it means
- Stephens (Wasatch Scientific) and Gubler (THREE) report predicted absorption for six named formulations, compared with curcumin.
- Methods
- One-hour assay; three replicates per product, two for control; differing test concentrations; spectrophotometric quantification.
- Limits to keep in view
- Cell-model predictions are not measured human absorption, clinical outcomes, or superiority to competing supplements. The source cautions against between-product comparisons. No matched formula without delivery technology was tested. Do not promise 3–9.5 times greater benefit or absorption in people.
- Source check
- All three PDF pages read · 25 October 2026
Manufacturer-hosted in-vitro THLE-2 liver-cell report2023-09 (2309.V1.US-EN)
Four formulas explored for gene-expression activity
The report detected formula-specific changes in selected gene transcripts, offering a starting point for further research.
Study scope and limitations
- What was studied
- Four historical formulas; Vitalité capsule portion only; no Purifí or Imúne. Test concentrations 50 or 150 micrograms/mL; triplicate assays.
- What it means
- Paul Davis, Catherine Davis and Dan Gubler tested Vitalité, Revíve, Éternel and Collagène with qRT-PCR after 24-hour exposure.
- Limits to keep in view
- RNA expression is not direct proof of DNA methylation, gene repair, human longevity, blood sugar control, cognition or weight loss. No formula revision/lot bridge is supplied. Charts and tables contain inconsistent Collagène signs and unusually expressed negative percentages; avoid decorative percentage claims. Potential overlap with the 2025 paper is unresolved, so do not count both as independent replication.
- Source check
- Eight-page PDF read, including methods and result tables · 25 October 2026
Journal research article; in-vitro gene-expression study2025-01-14
A published study of four formulas in liver cells
The publisher reports product-specific changes in six measured transcripts after 24 hours in cultured THLE-2 cells.
Study scope and limitations
- What was studied
- Results name Vitalité, Revíve, Éternel and Collagène; abstract methods mistakenly list both 'Reviv' and 'Revíve'. Current formula equivalence unverified.
- What it means
- Catherine E. Davis, Paul F. Davis and Daniel A. Gubler published Epigenetic effects of four nutraceutical products on human liver cells in Functional Foods in Health and Disease 15(1).
- Limits to keep in view
- This concerns cells exposed to products, not people taking supplements. It establishes neither clinical benefit nor GLP THREE effects. Full-paper methods, numerical results, funding and conflict disclosures could not be checked in this audit. Avoid presenting the abstract's broad health conclusions as proven outcomes.
- Source check
- Publisher metadata and complete abstract checked; full paper not reviewed · 25 October 2026
In-vitro THLE-2 liver-cell assay; one-page company report2024-03 (2403.V1.US-EN)
Revíve tested for superoxide activity
At 150 micrograms/mL, the table reports 35.87% inhibition in this laboratory assay.
Study scope and limitations
- What was studied
- Revíve historical formula; revision and lot unspecified; cultured cells only.
- What it means
- Gubler and Davis evaluated Revíve at 50 and 150 micrograms/mL using a WST-1 assay with absorbance at 450 nm.
- Limits to keep in view
- No human clinical outcomes or pharmacokinetic data are supplied to substantiate the report's oral-exposure comparison. Sample size and full statistical analysis are absent. The low-dose row reports 3.98% inhibition despite higher mean absorbance than activated control, an unresolved reporting concern. Do not translate this into promises of pain relief, lower inflammation in people or exercise recovery.
- Source check
- Complete one-page PDF read · 25 October 2026
The 2023 report and 2025 article may overlap. They are not counted here as independent replication. These findings are not transferred to GLP THREE or Visage.
03 · MANUFACTURING RECORDS
Read the credential.
Check the date.
Original copies identify a named holder, a scope and a displayed expiry. Dates below are assessed against the review date, 25 October 2026.
ORIGINAL SOURCE PACKET · 17 PAGES
Open original certificate packetManufacturing certifications
Inspect the issuing body and full document directly. No recreated certificate, approval seal or badge is used.
DISPLAYED EXPIRY2026-10-29Within displayed date at review
DISPLAYED EXPIRY2026-12-24Within displayed date at review
DISPLAYED EXPIRY2026-11-24Within displayed date at review
DISPLAYED EXPIRY2026-01-17Past displayed expiry at review
DISPLAYED EXPIRY2026-03-07Past displayed expiry at review
DISPLAYED EXPIRY2026-12-31Within displayed date at review
CSB’s NSF/ANSI copy shows expiry on 29 October 2026 and needs a renewed check before any later publication. Dates on copies do not independently establish present standing or renewal. Facility credentials are not product-lot testing or proof of efficacy; GMP for Sport is different from finished-product Certified for Sport. Halal coverage is limited to the products expressly named.
THE SOURCE IS PART OF THE STORY
Explore with confidence.
Ask good questions.
Read current product labels alongside these records. The full journal PDF was not reviewed in this audit; its publisher metadata and complete abstract were checked. Some image-only certificate pages were not visually verified.